14-3-3 zeta anticorps

N° du produit ABIN272122

Cet anticorps Lapin Polyclonal détecte spécifiquement 14-3-3 zeta dans WB, IF et IHC (p). Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 2+ publications.

Aperçu rapide pour 14-3-3 zeta anticorps (ABIN272122)

Antigène: 14-3-3 zeta (YWHAZ)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp 14-3-3 zeta est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-14-3-3 zeta anticorps

Specificité: This antibody detects endogenous levels of 14-3-3 ζ/δ protein. (region surrounding Trp228)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Références pour anticorps anti-14-3-3 zeta (ABIN272122)

Ihle, Trautmann, Kuenstlinger, Huss, Heydt, Fassunke, Wardelmann, Bauer, Schildhaus, Buettner, Merkelbach-Bruse: "miRNA-221 and miRNA-222 induce apoptosis via the KIT/AKT signalling pathway in gastrointestinal stromal tumours." dans: Molecular oncology, Vol. 9, Issue 7, pp. 1421-33, (2016) (PubMed).

Nagappan, Park, Park, Hong, Yumnam, Lee, Kim, Kim, Lee, Lee, Cho, Lee, Won, Cho, Kim: "Helicobacter pylori infection combined with DENA revealed altered expression of p53 and 14-3-3 isoforms in Gulo-/- mice." dans: Chemico-biological interactions, Vol. 206, Issue 2, pp. 143-52, (2013) (PubMed).

Détails sur 14-3-3 zeta

Antigène: 14-3-3 zeta (YWHAZ)

Autre désignation: 14-3-3 Protein zeta/delta

Sujet: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ

Poids moléculaire: approx. 28 kDa

ID gène: 7534

NCBI Accession: NP_001129171

UniProt: P63104

Pathways: Apoptose, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location